Patterning and Plant Development
Prof. Jim Haseloff, University of Cambridge.
These six lectures cover some striking features of biological self-organisation and morphogenesis using examples from the model plant, Arabidopsis thaliana. Plant cells are immobile, constrained by a rigid cell wall – yet plant development is plastic and indeterminate. Communication between neighbouring cells controls plant cell fate, and plays a major role in shaping plant growth.
Download lecture notes: (6.4 MB)
Online slide galleries:
Lecture 1 slide show Plant architecture and embryogenesis.
Lecture 2 slide show Polarity and auxin flow.
Lecture 3 slide show Regulation of gene expression by auxin.
Lecture 4 slide show Patterning of indeterminate growth.
Lecture 5 slide show Formation and specification of lateral organs.
Lecture 6 slide show Morphogenesis and engineering of plant systems.
Printouts of slides (PDF format):
Lecture 1 slides Plant architecture and embryogenesis.
Lecture 2 slides Polarity and auxin flow.
Lecture 3 slides Regulation of gene expression by auxin.
Lecture 4 slides Patterning of indeterminate growth.
Lecture 5 slides Formation and specification of lateral organs.
Lecture 6 slides Morphogenesis and engineering of plant systems.
Practical Laboratory: Patterning Processes - From Chemistry to Plants
Links to details of the Practical: Details of the practical including references
Extract from the Philip Ball book: “Shapes - Nature’s Patterns: a tapestry in three parts” is provided as reference material. This contains a non-technical description of the processes at work in this complex set of chemical reactions.
Self-organization in precipitation reactions far from the equilibrium: Elias Nakouzi and Oliver Steinbock, Sci Adv 2 (8), e1601144. DOI: 10.1126/sciadv.16011442016. 2016.
Below: Timelapse movie of B-Z reaction in the Practical Laboratory (movie: Kasey Markel), Gemma of Marchantia polymorpha at germination.
Mechanisms in Plant Development
Mechanisms in Plant Development, Ottoline Leyser & Stephen Day, Blackwell Science, UK, 2002.
Principles of Development
Further reading: Nature's Patterns: A Tapestry in Three Parts
Lecture review materials:
Field guide to plant model systems. Chang, C., Bownam, J.L. and Meyerowitz, E.M. Cell 167:325-339 (2016).
Early plant embryogenesis - dark ages or dark matter? Bayer, M., Slane, D. and Jürgens, G. Curr. Opinion in Plant Biology, 35:30-36 (2017).
Embryogenesis - the humble beginnings of plant life. Smet, I. D., Lau, S., Mayer, U., & Jurgens, G. The Plant Journal : For Cell and Molecular Biology, 61:959-70 (2010).
Lecture 2: Polarity and auxin flow.
Auxin: A major regulator of organogenesis. Bohn-Courseau, I. Comptes Rendus Biologies, 333:290-6 (2010).
Polar targeting and endocytic recycling in auxin-dependent plant development. Kleine-Vehn, J. and Friml, J. Ann. Rev. Cell Dev. Biol. 24:447-473, (2008).
The march of the pins: Developmental plasticity by dynamic polar targeting in plant cells. Grunewald, W., & Friml, J. The EMBO Journal, 29:2700-14 (2010).
Lecture 3: Regulation of gene expression by auxin.
Mechanisms of auxin signalling. Lavy, M. and Estelle, M. Development 143:3226-3229, (2016).
Transcriptional responses to the auxin hormone. D. Weijers and D. Wagner, Annual Rev. Plant Biol. 67:21.1–21.36 (2016).
Structural biology of nuclear auxin action. D.C. Dinesh, L.I.A. Calderón Villalobos and S. Abel, Trends in Plant Science, 21:302-315 (2016).
Building a plant: cell fate specification in the early Arabidopsis embryo. Colette A. ten Hove, Kuan-Ju Lu and Dolf Weijers. Development 142:420-430 (2015).
Lecture 4: Patterning of indeterminate growth.
Twenty years on: The inner workings of the shoot apical meristem, a developmental dynamo. Barton, M. K. Developmental Biology, 341:95-113 (2010).
CLAVATA-WUSCHEL signalling in the shoot meristem. Somssich, M., Byoung, J., Rüdiger, S. and Jackson, D. Development 143:3238-3248 (2016).
Lecture 5: Formation and specification of lateral organs.
Perspectives on leaf dorsoventral polarity. Szakonyi, D., Moschopoulos, A., & Byrne, M. E. Journal of Plant Research, 123:281-90 (2010).
Floral organ identity: 20 years of ABCs. Causier, B., Schwarz-Sommer, Z., & Davies, B. Seminars in Cell & Developmental Biology, 21:73-9 (2010).
Lecture 6: Morphogenesis.
Modeling plant growth and pattern formation. Jonsson, H., & Krupinski, P. Current Opinion in Plant Biology, 13:5-11 (2010).
Stochasicity in the symmetric division of plant cells when the exceptions are the rule. Besson, S. and Dumais, J. Frontiers in Plant Science 5:1-4, (2014).
Engineering quantitative trait variation for crop improvement by genome editing. Rodriguez-Leal, D; Lemmon, ZH; Man, J; Bartlett, ME and Lippman, ZB. 2017. Cell 171, 470-480.
(Click links to download PDF files)
Background reading about Arabidopsis
Arabidopsis thaliana as a model organism in systems biology. Norman, J. M. V., & Benfey, P. N. Wiley Interdisciplinary Reviews. Systems Biology and Medicine, 1:372-9 (2009).